Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Williams, S. C.
Right arrow Articles by Beatty, T.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Williams, S. C.
Right arrow Articles by Beatty, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

JOURNAL OF PLANKTON RESEARCH | VOLUME 17 | NUMBER 11 | PAGES 2037-2047 | 1995
© Oxford University Press

research-article

A new staining technique for dual identification of plankton and detritus in seawater

Samanthia C. Williams, Peter G. Verity and Thomas Beatty

Skidaway Institute of Oceanography 10 Ocean Science Circle, Savannah, GA 31411, USA

Received on March 28, 1995; accepted on July 11, 1995 A new method is described that employs dual staining and epifluorescence microscopy for the identification of plankton and detritus in seawater. The staining technique uses the fluorochromes propidium iodide (PI) and 4'.6-diamidino-2-phenylindole (DAPI) in conjunction with UV excitation. PI/DAPI staining identifies living matter distinct from non-living matter by selectively staining detritus a deep orange color while cells are either blue-white or pink, depending upon stain and cell type. Comparison of this technique with existing epifluorescence techniques and staining reagents indicates that PI/DAPI offers an improved approach for Staining detritus and plankton when evaluation of both is desired. This step is a prerequisite for quantitation of the volumes of detritus and plankton, preliminary to independent estimates of their carbon and nitrogen pools in aquatic ecosystems.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
N. Blackburn, A. Hagström, J. Wikner, R. Cuadros-Hansson, and P. K. Bjørnsen
Rapid Determination of Bacterial Abundance, Biovolume, Morphology, and Growth by Neural Network-Based Image Analysis
Appl. Envir. Microbiol., September 1, 1998; 64(9): 3246 - 3255.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.