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JOURNAL OF PLANKTON RESEARCH | VOLUME 7 | NUMBER 5 | PAGES 703-714 | 1985
© Oxford University Press

research-article

Sample preconditioning for measurement of fluorescence induction of chlorophyll a in marine phytoplankton*

Stephen S. Bates

Department of Fisheries and Oceans, Marine Ecology Laboratory, Bedford Institute of Oceanography Dartmouth, Nova Scotia, Canada B2Y 4A2

Received on January 1, 1985; accepted on June 1, 1985

Conditions for measuring fluorescence induction curves (time-scale ms) of in vivo chlorophyll a were studied using cultures of Dunaliella tertiolecta Butcher (Chlorophyceae) and of Thalassiosira pseudonana Hustedt (3H) (Bacillariophyceae), and samples of natural phytoplankton populations from the Grand Banks. The area above the fluorescence induction curve (ADCMU) and the maximum fluorescence intensity (Fmax) measured in the presence of 3-(3,4-dichlorophenyl)-1, 1-dimethylurea (DCMU) were computed by microcomputer. Cells must be ‘conditioned’ or ‘adapted’ prior to obtaining a fluorescence induction curve; dark-adaptation resulted in a lower ADCMU and Fmax than did adaptation in far-red (720 nm) light, and was the conditioning method chosen. ADCMU and Fmax increased linearly with increasing irradiance up to 32.8 W m–2 the highest actinic irradiance available. Information on the light history of D. tertiolecta was obtained by following the time-course of change in ADCMU and in Fmax for cells exposed for 10 min to far-red or to blue light. The rise-time of the fluorescence induction curve and values of Fmax were greater for samples of D. tertiolecta concentrated onto glass-fiber filters than for liquid samples, however, values of ADCMU for filtered and liquid samples were not significantly different. Samples of Grand Banks phytoplankton collected onto glass-fiber filters and frozen for 28 d exhibited a significant decrease in Fmax and in ADCMU relative to the same freshly-filtered samples. Filtration and freezing of samples is not recommended.

*This paper is the result of a study made at the Group for Aquatic Primary Productivity (GAP). Second International Workshop held at the National Oceanographic Institute. Haifa. Israel in April–May 1984.


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