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JOURNAL OF PLANKTON RESEARCH | VOLUME 9 | NUMBER 5 | PAGES 945-953 | 1987
© Oxford University Press

research-article

Rapid-freeze preservation minimizes radioisotope leakage from zooplankton in feeding experiments

Michael E. Sierszen1 and Carl J. Watras1,2

1Center for Limnology, University of Wisconsin Madison, WI 53706 2Bureau of Research, Wisconsin Department of Natural Resources Madison, WI 53711, USA

Received on October 21, 1986; accepted on June 10, 1987 Preservation of labelled zooplankton by rapid freezing on dry ice minimizes loss of 14C and 32P. 14C retention in frozen samples approximates 100% even after 24 h storage. This is a major improvement over retention in chemical preservatives such as ethanol. Isotope loss in ethanol is rapid (on a time scale of minutes) and extensive (up to 72%). 32P appears to be more labile than 14C, and exposure of labelled animals to liquids must be minimized to effectively conserve this isotope. 32P retention is then improved, but may vary between 75 and 100%. We urge continued caution in feeding experiments using 32P, especially when dual labelling to measure selectivity.


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